Here is a Nikon 50i Trinocular Microscope
Setup for Brightfield with Phase Contrast
This Nikon 50i Microscope is in good working order with all aspects tested to be working fully.
Microscope optics have been cleaned professionally by our technicians under a class 10,000 clean room hood.
Please see the exact video and photos below.
Testing we performed:
The Nikon 50i Microscope passed all tests and was found to be fully functional:
Tested focal adjustment
Tested halogen lamp fully
Tested mechanical XY stage to limits
Tested mechanical objective turret
Tested objectives for clarity
Tested eyepieces for clarity
Tested phase contrast prisms
Tested live feed connection
During testing and cleaning, this microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the mechanical components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Nikon Eclipse 50i Microscope
Trinocular Tube Head
(2) CFI 10x/22 Eyepieces
Prisms / Condenser:
Phase Contrast 0.90 Dry Condenser
Prisms: Ph1, Ph2, Ph3
Built in Halogen Lamp
6V 30W Halogen Lamp
Mechanical XY Stage w/ Specimen holder
54 x 78 Travel
6-position Mechanical Nosepiece
20x/0.75 CFI Plan Apo VC (this is a high quality Nikon APO CFI60 Objective, it was originally installed in a custom piece of equipment where manufacturer did not want to show that they used Nikon objectives)
40x/0.55 Ph1 ADL LWD
Photo / Port Splits:
100% Eye or 100% Camera
AmScope MU300 3.1MP CMOS Camera
Computer and Software:
Dell Latitude i5 running Windows 7 Pro with AmScope software
Communication and power cables.
Configuration and Setup:
Illumination Type: Brightfiled with Phase Contrast
Objectives: 20x/0.75 CFI Plan Apo VC, 40x/0.55 Ph1 ADL LWD
Light Sources: 6V 30W Halogen Lamp
Stage Type: Mechanical XY Stage
This Nikon 50i Microscope is currently set up for Brightfield viewing with Phase Contrast.
The MU300 AmScope CMOS digital color camera is capable of taking detailed digital images.
The Leica system includes a Dell Latitude i5 laptop to control the AmScope software for live feed images.
This video begins with a full front view of the Nikon 50i Microscope powered on and running.
The camera pans from right to left for a more detailed look.
Next we see the AmScope live feed with a demonstration via the mechanical stage.
Lastly we see the Phase Contrast prisms and what it looks like with matching objective over the live feed.
A final front view of the Nikon 50i Microscope will end this video.
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