Here is a Nikon Ti-E Fluorescence Motorized DIC Polarization Phase Contract Microscope
Setup for Brightfield with Fluorescence, DIC, Polarization, Phase Contract studies.
This Nikon Ti-E Fluorescence Motorized DIC Polarization Phase Contract Microscope is in good working order with all aspects tested and working fully.
Microscope optics have been cleaned professionally under a class 10,000 clean room hood.
Please see the exact video and photos below.
Testing we performed:
The Nikon Ti-E Fluorescence Motorized DIC Polarization Phase Contract Microscope passed all tests and was found to be fully functional:
Tested halogen lamp and source fully: PASSED
Tested EXFO lamp and source fully: PASSED
Tested port splitter to split between all ports: PASSED
Tested connection to computer: PASSED
Tested motorized XY stage to limits: PASSED
Tested motorized focal adjustment to limits: PASSED
Tested objectives for clarity: PASSED
Tested eyepieces for clarity: PASSED
Tested phase contrast condenser and prisms: PASSED
Tested DIC condenser and objective prisms: PASSED
Tested fluorescence light path and shutters: PASSED
Tested fluorescence filter cubes and turret: PASSED
Tested all power switches and adjustments: PASSED
Tested virtual control of motorized components: PASSED
During testing this Nikon Ti-E Fluorescence Motorized DIC Polarization Phase Contract Microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the motorized components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Nikon Eclipse Ti-E Inverted Microscope
TI-TD B Binocular Head
(2) CFI 10x/22 Eyepieces
Prisms / Condensers:
TI-CT-E 5-position Motorized DIC Condenser Head LWD 0.52
Condenser Prisms: PhL, DIC M
D-LH/LC Illumination Arm
TI-FL Fluorescence Light Path
TI-FLC-E 6-position Motorized Filter Cube Turret
HQ TR (Red)- EX 560/55nm DM 595lp EM 645/75nm
ET GFP (Green)- EX: 470/40nm DM: 495nm EM: 525/50nm
UV-2E/C (UV)- EX: 360/40nm DM: 400nm EM: 460/50nm
40x/0.60 Plan Fluor ELWD DIC M Objective
40x DIC objective prism
DIC M condenser prism
D-LH/LC 12V 100W Halogen Lamp w/ UN2-PSU100 100W Halogen Power Supply
EXFO X-Cite 120 Metal Halide Lamp w/ C-HGFIA Culminating adapter, liquid light guide
TI-S-E Motorized Stage
TI-S-CON Control Box
TI-S-EJOY XYZ Joystick
6-position Motorized Nosepiece
4x/0.13 Plan Fluor PhL DL
20x/0.75 CFI Plan Apo VC (this is a high quality Nikon APO CFI60 Objective, it was originally installed in a custom piece of equipment where manufacturer did not want to show that they used Nikon objectives)
40x/0.60 Plan Fluor ELWD DIC M
Photo / Port Slits:
Left 100% or Left 80% Head 20% or Right 100% or Head 100%
AmScope MU1003 10.0MP USB 3.0 Colored CMOS camera
Computer and Software:
Dell Latitude i5 running Windows 7 Pro with AmScope software and MicroManager software, and Nikon Ti Control Software
XY Stage, Z-Axis, Light Path Switching, Nosepiece, Fluorescence Turret, Condenser
Communication and power cables.
Configuration and Setup:
Illumination Type: Brightfield, Fluorescence, Phase Contrast, DIC, Polarization
Objectives: 4x/0.13 Plan Fluor PhL DL, 20x/0.75 CFI Plan Apo VC, 40x/0.60 Plan Fluor ELWD DIC M
Light Sources: 12V 100W Halogen Lamp, EXFO X-Cite 120W Metal Halide
Stage Type: Motorized XY
This Nikon Ti-E Fluorescence Motorized DIC Polarization Phase Contract Microscope is currently set up for Brightfield with Fluorescence, Phase Contrast, DIC and Polarization studies.
The MU1003 10MP camera is capable of taking detailed digital images.
With 4x, 20x, 40x objectives along side 10x eyepieces and in internal magnification of 1x- 1.5x the total magnification of this scope is 600x
This video begins with a full front view of the Nikon Ti-E Fluorescence Motorized DIC Polarization Phase Contract Microscope powered up and running.
The camera pans from right to left for a more detailed look. We see the MicroManager and AmScope software information alongside the live feed.
Next, we see motorized features as they are controlled virtually: the stage, focal adjustment, condenser, nosepiece, and fluorescence turret.
A final front view of the Nikon Ti-E Fluorescence Motorized DIC Polarization Phase Contract Microscope will end this video.
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