For Sale Molecular Devices ImageXpress Micro Confocal HT.ai High-Content Laser Imaging System Including Robot w/ Environmental Control, Water Immersion & Transmitted Light Options
• Fully Functional
• Serviced & in Stock
• Current Model
• Good cosmetic condition
• Technical sales consultation available
• WARRANTY
• Optional on-site installation available
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Here is what you will receive:
• Molecular Devices ImageXpress Micro Confocal HT.ai High-Content Imaging System
• ImageXpress power and options controller
• Installed emission Filters: DAPI, FITC, CFP, Texas Red, CY5, CY7, YFP
• Nikon Objectives:
- 4x/0.20 Plan Apo
- 10x/0.45 Plan Apo Lambda
- 20x/0.95 Water Apo Lambda S LWD
- 40x/1.15 Water Apo Lambda S LWD
• 89 North LDI-NIR EX light engine (Class 4 Laser)
• Wave Lengths: 405, 445, 470, 640, 520, 555, 730
• Nikon Illumination arm with Nikon 100W Halogen lamp (D-LH/LC)
• Live Cell Instrument FC-9 Gas Mixer
• Water Immersion Control Box
• Single Slide Holder
• Computer running MetaXpress 6.7.2 with license dongle
• Automation Computer running Biosero Green Button Go v7.1.4
• Brooks PreciseFlex 400 sample handler robot
• (4) Molecular Devices 20-position plate hotels
• Barcode Tray position and swap position
• Emergency Stop button
• Laboratory table with predefined mount points and mount hardware
• Monitor, mouse, keyboard
• Communication and power cables
ImageXpress Confocal HT.ai HCI System Configuration:
• Camera: Andor Zyla sCMOS
• Spinning disk geometry: 50 um slit and 60 um pinhole (dual disk)
• High-speed laser autofocus with integrated image autofocus option
• Light Source: 89 North LDI light engine & 100W Halogen lamp
• Linear encoded voice coil-driven X, Y, and Z stages with better than 25 nm resolution
• 4-position automated objective changer
• Objectives:
- 4x/0.20 Plan Apo
- 10x/0.45 Plan Apo Lambda
- 20x/0.95 Water Apo Lambda S LWD
- 40x/1.15 Water Apo Lambda S LWD
• 5-position software-selectable dichroic filter wheel
• 8-position software-selectable emission filter wheel
• Filters Installed: DAPI, FITC, CFP, Texas Red, CY5, CY7, YFP
• Sample compatibility: slides and one to 1536-well microplates, round or flat bottom, low to high profile
• Throughput: >160K wells/day confocal, >200K wells/day widefield
Brooks PreciseFlex 400 Configuration:
• Configured Options: 750mm Z travel and Extended Reach Arm
• Range of motion & resolution:
• - J1 (Z) axis: 750mm
• - J2 axis: ± 90 degrees
• - J3 axis: ± 167 degrees
• - J4 axis: +/- 970 degrees
• Horizontal Reach (Extended Horizontal Reach, with included Servo Gripper) 734 mm
• Spring gripper force: 2-23 Newtons closing, 2-10 Newtons opening, 7 Newtons power off
• Resolution: 10 microns typical
• Repeatability: +/- 0.090 mm overall in x,y & z directions at 18-22C
• Maximum acceleration: 2500mm/sec2 with 500 gram payload
• Maximum speed: 500 mm/sec Cartesian, 360 degrees/sec J2, 720 degrees/sec J3, J4
• Labware: SBS plates can be picked in landscape and portrait orientation.
• Weight: 30kg
Installed OPTIONAL System Features:
Environmental Control:
• Multi-day, live cell time-lapse imaging
• Provides appropriate atmospheric conditions. LCI FC-9 Gas Controller supports 5-10% CO₂
• Mimics physiological environment (30-40 DegC ± 0.5 DegC)
• Controls humidity and minimizes evaporation (0.5 μL/well/hour for 96- or 384-well formats)
Water Immersion Option:
• Water-immersion controller maintains a stable water layer for high-NA imaging of live or thick samples.
• Water-immersion objectives (20x/0.95, 40x/1.15) improve resolution and reduce aberrations in aqueous 3D specimens.
Transmitted Light Option:
• High-contrast imaging, where unstained cells are easily viewed or separated from the background
• Nikon Illumination arm with Nikon 100W Halogen lamp
• Ideal for non-fluorescent histochemically stained samples
• Fluorophore-independent morphology visualization with fluorescent imaging overlay
Software Modules:
MetaXpress 6.7 Acquisition and Offline Analysis Software included foundational tools:
• Time-series acquisition and analysis
o Integrated with Acquisition and Analysis Wizards
• Z-series acquisition
o Acquisition integrated with the Acquisition Wizard
• Z-series analysis
o Analysis integrated into the workflow
o Compatible with MetaXpress PowerCore software
• Customized analysis
o Integrated Custom Module Editor
• Transmitted light segmentation
o Simple step cards are available with the Custom Module Editor
• Asynchronous kinetics
o Built into the Acquisition Wizard
• Basic curve fitting
o Included with the MetaXpress software
• Peak analysis
o Included with the MetaXpress software
• Basic 3D acquisition setup tools
o Includes Z-stack capture, 2D projection options
o Interactive GUI for Z settings
Included OPTIONAL Application Modules:
Customization/Options:
Other objectives might be available; please inquire with our sales team.
Testing performed by our in-house service team:
The ImageXpress Confocal HT.ai HCI System passed all tests and was found to be fully functional:
• We initialized the ImageXpress Micro Confocal System.
• We established communication with the software.
• We checked the clarity of the objectives.
• We tested the laser autofocus functionality.
• We ensured clarity in all installed filters.
• We tested the laser interlock safety shutoff.
• We verified the functionality of the 89 North LDI
• We performed multi-well acquisitions.
• We performed multi-channel slide acquisitions with image stitching.
• We tested the functionality of the emission and dichroic filter wheels.
• We conducted spatial calibration.
• We confirmed the operation of the FC-9 Gas Controller.
• We confirmed camera clarity and functionality.
• We verified full X- and Y-stage movement.
• We confirmed the full Z movement.
Brooks PreciseFlex 400 testing steps performed:
• We established communication with the Green Button Go for program development and testing.
• We established communication between the ImageXpress control PC and the Automation PC
• We executed the homing cycle, confirming accurate and consistent motion alignment.
• We ran multi-plate acquisitions to ensure smooth and reliable operation.
• We performed a full calibration to verify the precision of the system’s positioning.
• We tested collision detection and confirmed the system stopped immediately upon detection.
• We verified the emergency stop control.
• We monitored for joint position errors to ensure accurate joint movement and error-free operation.
• We assessed the joint envelope to validate the full range of motion and correct limits.
• We tested the gripper’s open/close functions, ensuring precise and responsive action.
• We checked the internal CMOS and servo encoder batteries, ensuring they functioned properly for consistent performance.
Instrument Overview:
• Molecular Devices ImageXpress Micro Confocal HT.ai delivers high-content confocal imaging with laser-based optical sectioning.
• Integrated environmental control supports stable live-cell imaging with regulated temperature, humidity, and CO₂.
• Water-immersion Nikon objectives provide high numerical aperture imaging for improved resolution and signal capture.
• The system includes transmitted-light imaging via a Nikon halogen illumination arm for structural visualization.
• Automated plate handling is enabled by a Brooks PreciseFlex 400 robot with multiple 20-position plate hotels.
• Designed for high-throughput, unattended operation, the platform supports fully automated imaging workflows.
Instrument Application:
• High-content imaging and quantitative analysis of live or fixed cells
• Multiplexed fluorescence assays for multi-parameter cellular readouts
• 3D imaging of spheroids, organoids, and complex tissue models
• High-throughput phenotypic screening for drug discovery
• Toxicity, viability, proliferation, and cell-health assessments
• Subcellular structure visualization and tracking over time
• Automated image acquisition for large plate formats and screening workflows
The Key Features:
• High-speed confocal imaging
• Widefield and confocal modes
• Environmental control
• Water-immersion optics support
• Multi-channel fluorescence
Why do so many customers choose to order from us?
Our Advantage
• We have experience servicing and repairing these instruments.
• This instrument goes through all the described service steps before shipping. see the questions section below
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Technical sales consultation available
• Remote installation and instrument operation training may be available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)
Our Guarantee
• Warranty
• You get after-sales technical support
• Customer references are available
• The instrument will be fully functional!
• Smooth process from the initial quote to order fulfillment
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This will ship via freight. Please contact us with your zip code to obtain a shipping quote.
*LA* Large Crate
790#
PLEASE NOTE THAT THE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE
Q: Can I view this instrument in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.
Q: What about the warranty?
A: The instrument comes fully warranted for both parts and labor.
Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to familiarize you with this instrument.
Q: What should I watch out for when buying a used/pre-owned instrument from another lab?
A: Ensure that the instrument is in good working condition with no damage or wear that could affect its performance. Ask for details about testing steps performed, video demo, and test screenshots.
Q: Do you have more than one of these instruments on hand?
A: We generally carry multiple instruments on hand, along with a variety of spare parts.
Q: This procurement is for a University or College. Are purchase orders with NET terms accepted?
A: We accept purchase orders from all major US and Canadian universities.
Q: How fast will you ship?
A: We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.
Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.
Q: How do you pack? Have you previously packed and shipped the fragile laboratory instruments?
A: We have extensive experience shipping high-end and complex laboratory instruments in the US and Internationally.
Q: Do you ship Internationally?
A: Yes! We have shipped to multiple continents and over 30 countries.
Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new instrument, you will save 60% or more and have plenty of funds left over for an additional instrument or two.
TO VIEW ADDITIONAL PHOTOS, VIDEOS AND VERY DETAILED INFORMATION SUCH AS TEST STEPS PERFORMED, CONFIGURATION, ETC., PLEASE VISIT OUR WEB SITE. TO GET THERE PLEASE CLICK ON “WEBSTORE”; TO YOUR RIGHT AND YOU WILL SEE LINK TO OUR SITE IN THE MIDDLE OF THE SCREEN. THEN USE LAST 5 DIGITS OF MPN NUMBER IN THE SEARCH FIELD TO LOCATE YOUR ITEM.
DO NOT HESITATE TO CALL US OR EMAIL US WITH ANY QUESTIONS YOU MIGHT HAVE.
ALL OF OUR ITEMS ARE AVAILABLE FOR INSPECTION AT OUR FACILITY.
Return Policy
Items are sold as-is with no returns or refunds available unless explicitly stated.
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