For Sale BIO-RAD ZE5 Flow Cytometer

For Sale Bio-Rad ZE5 Cell Analyzer Flow Cytometer (5)Lasers/(27)Colors/(29)Detectors Unit 2

CURRENTLY SOLD OUT, IF YOU ARE INTERESTED PLEASE SUBMIT YOUR INTEREST AND WE WILL UPDATE YOU ONCE INSTRUMENT IS BACK IN STOCK.

• Fully Functional
• Serviced & in Stock
• Technical sales consultation available
• Includes subscription to De Novo FCS Express 7 Analysis software
• CONTINENTAL US 90 DAYS WARRANTY & 45 DAYS INTERNATIONAL
• Optional on-site setup and training are available for customers within 2 hours of Boston, MA
• Optional remote installation support and training are available for non-local customers
• Optional extension to full 1-YEAR warranty within USA

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Here is what you will receive:
• Bio-Rad ZE5 Cell Analyzer Flow Cytometer
• Desktop with "Everest" Software version 3.2.12
• Monitor with Mouse, Keyboard
• All Communication and Power Cables
• Complimentary De Novo FCS Express analysis software (1-Year Subscription) RUO version
• Communication and power cables

Includes complimentary 1-year subscription to De Novo FCS Express 7 Analysis software
Enhancing your cytometer research capabilities with these key benefits:

• User-Friendly Interface: Navigate complex data analysis easily using FCS Express's intuitive design.
• Advanced Analysis Tools: Utilize sophisticated tools for in-depth data exploration and interpretation.
• Comprehensive Reporting: Generate detailed, customizable reports that are publication-ready.
• High Compatibility: Supports multiple file formats, including FCS, LMD, CSV, BD FACSDiva, Beckman Coulter files, Accuri C6 files, SPADE, Cytobank files, and TXT files.
• Enhanced Data Visualization: Create high-quality graphs and plots to visualize your data effectively
• With FCS Express 7, you will have the latest Research Use analysis software.

ZE5 Cell Analyzer Configuration:
Optics:(5) Lasers, (27) Colors, (29) Detectors:
• Forward Scatter Channel: 488 blue laser
• Second Forward Scatter Channel: 405 violet laser
• Laser Color Allocation (Laser/Channel Count): Blue/4, Red/4, Violet/7, UV/5, Yellow/7
• Fluorescence sensitivity: average MESF - FITC:<70; PE:<50; APC:<40
• Scatter sensitivity <0.5 μm FSC resolution with standard FSC detector
• Speed: up to 100,000 eps with all parameters enabled

Standard filter configuration shown here (Please inquire for exact configurations we have in stock)
• 355nm Ultraviolet Laser: B1 525/50, B2 670/30, B4 700 LP, B5 447/60, B7 387/11
• 405nm Violet Laser: C1 670/30, C2 720/60, C4 750LP, C5 460/22, C7 420/10, C8 615/24, C10 525/50
• 488nm Blue Laser: A1 488/10, A4 525/35, A5 593/52, A8 750LP, A10 692/80
• 561nm Yellow/Green Laser: C1 750LP, C2 670/30, C4 720/60, C5 589/15, C7 577/15, C8 640/20, C10 615/24
• 640nm Red Laser: B1 720/60, B2 775/50, B4 800 LP, B5 670/30

Fluidics:
• Loader: integrated sample loader with agitation and temperature control.
• Compatible labware:
• - 5 ml tubes (12 x 75 mm, 1-40 tubes per rack)
• - 1.5 ml microcentrifuge tubes (1-24 tubes per rack)
• - 96-well plates, 96 deep-well plates, 384-well plates
• - 1x 5 ml tube in the stat tube position (12x75 mm)
• Throughput <15 min for 96-well plate and <60 min for 384-well plate in high-throughput mode
• Sample flow rates 0.1–3.5 ul/sec (6–210 ul/min) (standard mode); 0.5–2.5 ul/sec (30–150 ul/min) (high-throughput mode)
• Bulk fluid system for 4L Carboys

Customization/Options:
• Optional accessories include tube racks, an external bulk fluid cart, or optical configurations. (Please inquire for available inventory)

Testing performed by our in-house service team:
The ZE5 Cell Analyzer passed all tests and was found to be fully functional:
• We test the power and initialization.
• We verify communication between the Everest software and the ZE5.
• We inspect the electrical system.
• We complete the system startup process without errors.
• We complete the system shutdown process without errors.
• We create a new experiment with plots and gating.
• We run the cleaning and decontamination protocols.
• We thoroughly clean and inspect the fluidics system.
• We test the operation and function of the interior lighting.
• We test the function of the Loader.
• We test the ZE5 sample loader's operation.
• We test the operation of both external fluidics carts (DI and waste) if included.
• We thoroughly clean and inspect the optical system.
• We check laser power and stability.
• We adjust alignment using our alignment beads.
• We complete quality control (QC) testing using our multi-peak calibration beads.

Instrument Overview:
• The Bio-Rad ZE5 Cell Analyzer features five lasers for versatile analysis of various samples across 27 colors.
• Features dual FSC and high acquisition rates of up to 100,000 events/second.
• Incorporates external DI water and waste fluidics carts, enhancing operational fluid management capabilities.
• The ZE5 Cell Analyzer's innovative fluidics system minimizes carryover and maximizes analytical throughput.
• The sample loader accommodates multiple formats, with agitation and temperature control.
• With easy interchangeability between sample formats, the instrument enhances user efficiency in busy lab environments.

Instrument Application:
• High-throughput screening for drug discovery
• Immunophenotyping of immune cell populations
• Analysis of cell cycle and apoptosis
• Multiparameter analysis of cell signaling pathways
• Characterization of stem cells and their differentiation
• Detection of rare cell populations in clinical samples

The key features:
• Five lasers for enhanced multicolor analysis
• 27 colors for diverse application flexibility
• High-throughput sample processing efficiency
• Integrated external DI water system for fluidics
• Versatile research applications.

De Novo FCS Express Software Features
• Data Import and Handling: Easily imports data from various flow cytometry instruments, supporting a wide range of file formats.
• Intuitive Interface: User-friendly interface with customizable dashboards for streamlined workflow management.
• Advanced Data Analysis: Provides comprehensive tools for complex data analysis, including statistics, gating, and kinetic analysis.
• Visualization Tools: Offers a variety of plotting options, such as histograms, scatter plots, and heat maps, for effective data visualization.
• Automated Reporting: Facilitates the creation of detailed reports with integrated data and graphics, automating repetitive tasks.
• Real-Time Data Updates: Supports real-time data analysis and visualization, enabling immediate insights and decision-making.
• Collaboration Features: Allows sharing of data and analysis results with team members through secure cloud-based platforms.
• Integration Capabilities: Seamlessly integrates with other laboratory software and databases for efficient data management and workflow integration.

Benefits of the Complimentary DeNovo FCS Express Software
• Enhanced Data Management: Streamlines data import, handling, and analysis, improving overall data management efficiency.
• User-Friendly Design: Intuitive interface design ensures ease of use, reducing the learning curve and increasing productivity.
• Comprehensive Analysis Tools: Advanced analysis tools allow for in-depth examination of complex data sets, leading to more accurate results.
• Flexible Visualization Options: Wide range of plotting options helps in effectively visualizing data, facilitating better understanding and presentation.
• Time-Saving Automation: Automated reporting and analysis features save time and reduce manual effort, enhancing workflow efficiency.
• Immediate Insights: Real-time data updates enable quick insights and decision-making, crucial for time-sensitive experiments.
• Collaboration and Sharing: Secure cloud-based collaboration features promote data sharing and teamwork, enhancing research collaboration.
• Seamless Integration: Integration capabilities with other software and databases ensure smooth workflow and data management.

Note: Subscription is for the Research Use Only (RUO) version.

CRITICAL TECHNICAL STEPS WE TAKE TO ENSURE INSTRUMENT PERFORMANCE & CUSTOMER SATISFACTION

They want to ensure the cytometer undergoes full fluidics system decontamination, flushing, and inspection to protect the valves and flow sensors from damage.
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They want to ensure the cytometer has no residual sheath salt buildup in the flow cell, which could skew scatter data and, and if improperly cleaned, can damage the flow cell.
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They want to confirm that all detectors, including forward scatter (FSC) and side scatter (SSC) detectors, can be aligned and calibrated for particle sizing and granularity resolution.
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They want assurance that baseline performance has been verified using standardized particles, with data or log files available for review.
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They want to confirm that electronic noise and EMI levels at the detector/amplifier stage remain within tolerance and will not distort signal fidelity.
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They want to ensure that no fiber optic cables are missing, misrouted, or defective, which can lead to diminished fluorophore excitation or signal dropout.
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They want to confirm that no power degradation or misalignment in laser paths is detected through precise mW-level measurement at the final optics output.
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They want to ensure that optical filters and dichroic mirrors have been inspected for delamination, spectral shift, or contamination that could compromise signal specificity.
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They want to ensure the sample flow rate is linear and stable across a full range of flow settings, verified with volumetric calibration and pressure profiling.
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They want to ensure the system passes automated QC and performance tracking with reproducible CVs for bead standards across multiple channels.
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They want to ensure the fluidics pump assembly does not exhibit pulsation, drift, or backpressure due to check valve wear or micro-leaks.
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They want peace of mind, knowing their instrument is backed by a warranty from a company that can stand behind it.

Our Advantage
• We have experience servicing and repairing these instruments.
• This instrument goes through all the described service steps before shipping. see questions section below
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Technical sales consultation available
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

Our Guarantee
• Warranty
• You get after-sales technical support
• Customer references are available
• The instrument will be fully functional!
• Smooth process from the initial quote to order fulfillment

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This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

PLEASE NOTE THAT THE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE

Q: Can I view this instrument in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.

Q: What about the warranty?
A: The instrument comes fully warranted for both parts and labor.

Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to familiarize you with this instrument.

Q: What should I watch out for when buying a used/pre-owned instrument from another lab?
A: Ensure that the instrument is in good working condition with no damage or wear that could affect its performance. Ask for details about testing steps performed, video demo, and test screenshots.

Q: Do you have more than one of these instruments on hand?
A: We generally carry multiple instruments on hand, along with a variety of spare parts.

Q: This procurement is for a University or College. Are purchase orders with NET terms accepted?
A: We accept purchase orders from all major US and Canadian universities.

Q: How fast will you ship?
A: We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.

Q: How do you pack? Have you previously packed and shipped the fragile laboratory instruments?
A: We have extensive experience shipping high-end and complex laboratory instruments in the US and Internationally.

Q: Do you ship Internationally?
A: Yes! We have shipped to multiple continents and over 30 countries.

Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new instrument, you will save 60% or more and have plenty of funds left over for an additional instrument or two.

TO VIEW ADDITIONAL PHOTOS, VIDEOS AND VERY DETAILED INFORMATION SUCH AS TEST STEPS PERFORMED, CONFIGURATION, ETC., PLEASE VISIT OUR WEB SITE. TO GET THERE PLEASE CLICK ON “WEBSTORE”; TO YOUR RIGHT AND YOU WILL SEE LINK TO OUR SITE IN THE MIDDLE OF THE SCREEN. THEN USE LAST 5 DIGITS OF MPN NUMBER IN THE SEARCH FIELD TO LOCATE YOUR ITEM.

DO NOT HESITATE TO CALL US OR EMAIL US WITH ANY QUESTIONS YOU MIGHT HAVE.

ALL OF OUR ITEMS ARE AVAILABLE FOR INSPECTION AT OUR FACILITY.

Return Policy

Items are sold as-is with no returns or refunds available unless explicitly stated.