For Sale EPPENDORF BioFlo 320 Eppendorf BioFlo 320 Dual Bioreactor System w/ 10L Vessels, MC 1200 Chiller, DASGIP OD4 & Exhaust Gas Modules

For Sale Eppendorf BioFlo 320 Dual Bioreactor System w/ 10L Vessels, MC 1200 Chiller, DASGIP OD4 & Exhaust Gas Modules

• Fully Functional
• Serviced & in Stock
• Current Model
• Technical sales consultation available
• CONTINENTAL US 120 DAYS WARRANTY & 60 DAYS INTERNATIONAL
• Includes remote instrument setup and operation training session

Bioreactors are configured to meet individual customer requirements (shown with a 10L glass autoclavable vessel)

We will be able to configure this bioreactor for either microbial production or bioprocessing

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Here is what you will receive:
• 2x Eppendorf Bioflo 320 Bioprocess Control Station (4 built-in Peri Pumps, Direct drive motors)
• 2x ELO Touchscreen Monitor w/ Arm Mount
• 2x 10L vessels (with a Heating/Cooling base) – Other vessel sizes maybe available
• 2x Stirring drives
• 2x Temperature Sensors
• 2x PH Sensors
• 2x DO (Dissolved Oxigen) Sensors
• 2x Level/Foam Sensors
• 2x Exhaust gas coolers (w/ quick connect tubing)
• 2x External Watson Marlow 120U Peristaltic Pump (with harness to operate through BioFlo320)
• 1x LAUDA Microcool MC 1200 Circulation chiller 115 V; 60 Hz (with supply/return tubing harness)
• 1x Dasgip OD4 Optical Density module
• 2x Optical Density sensors for 10L vessel
• 2x BlueInOne exhaust O2/CO2 sensors; w/ BlueVis software
• Desktop PC (Monitor, keyboard, mouse, network switch) with the BioCommand software
• All power and communication cables

• The DASGIP OD4 module enables real-time, non-invasive optical density monitoring in up to four vessels. With OD sensors, it tracks biomass growth during microbial processes, helping optimize feeding and harvest timing.

• The LAUDA Microcool MC 1200 Circulation chiller is ideal for temperature control in bioprocessing. When used with the Eppendorf BioFlo 320, it ensures stable thermal conditions for dual bioreactor operations, supporting reliable cell culture and fermentation. With up to 1.2 kW cooling capacity and a -10 to +40 DegC temperature range, it enhances process consistency and efficiency in R&D and scale-up applications.

• The BlueSens exhaust gas cooler with quick-connect tubing cools and dries bioreactor exhaust gases before they reach gas sensors, protecting them from heat and condensation. This setup enables accurate, real-time off-gas analysis (e.g., CO₂, O₂)

• The BioCommand software is designed to enhance your ability to monitor and control your fermentation and cell culture processes through your personal computer. It allows the integration of additional inputs. For example, once configured, the DASGIP OD4 module and Bioflo 320 Bioprocess Control Stations may be monitored by the BioCommand software as a supervisory control and data acquisition (SCADA) software as a complete system.

Bioreactor Vessel Configuration: (2x 10L vessels)
• Bioreactor: autoclavable, stainless-steel dished-bottom, direct drive
• Working volume range: 3.5 - 10.5 L
• Impellers: 2 x Rushton type
• Stainless Baffles
• Sparger Type: ringsparger
• Heated/Cooled base for temperature control

Reactor Customization/Options might be available upon request. Please inquire with our sales team.
• Different volume size vessels
• Choice of agitation impellers (pitched blade, marine, Rushton)
• Redox or CO2 sensors
• Vessel Scale
• Sparge and/or Overlay vessel attachment
• Level / Foam sensors
• Others accesories
Please note that, depending on the requested options, we may sometimes recommend a different model bioreactor that may better fit your application.

Testing performed by our in-house service team:
The BioFlo 320 bioprocess control system passed all tests and was found to be fully functional:
• We confirm communication between all units and the PC, including the BioFlo network, DASGIP USB COM, and BlueInOne COM ports.
• We verify the power supply and complete the system initialization process.
• We assess the functionality of the touchscreen display, confirming responsive and accurate input.
• We test the agitation motor and motor control, confirming smooth and stable operation at multiple RPM ranges.
• We test Pumps 1 through 4 to ensure reliable and accurate delivery rates, including external pumps added to the system.
• We verify the pH sensor readings using a calibration solution, confirming accurate output.
• We verify the dissolved oxygen (DO) sensor readings using a calibration solution, ensuring sensor accuracy.
• We tested OD using test solutions at our selected concentrations.
• We test the heating control system and verify temperature accuracy using a NIST-traceable calibrated probe.
• We test the gas output via the sparge line and verified gas control functionality.
• We test all add-on options and accessories.
• We perform additional testing as requested within reasonable parameters.

Instrument Overview:
• Eppendorf BioFlo 320 Dual Bioreactor System includes two 10L vessels with direct drive motors and Rushton impellers.
• Modular base units with integrated peristaltic pumps and support for left- and right-hand orientation setups.
• Gas mixing via TMFC with 4-gas capability and autoclavable push-connect fittings rated up to 10 psig.
• LAUDA MC 1200 chiller ensures stable temperature control using quick-connect supply and return tubing.
• DASGIP OD4 module enables real-time optical density monitoring with sensors for each 10L vessel.
• Exhaust gas coolers with quick-connect tubing protect analyzers during off-gas CO₂/O₂ monitoring and sterilization.

Instrument Application:
• Microbial fermentation for protein or enzyme production (e.g., E. coli, yeast)
• Biomass growth monitoring and optimization
• Parallel fed-batch process development
• Scale-up studies for biologics manufacturing
• Real-time metabolic profiling via off-gas analysis (CO₂/O₂)
• Temperature-sensitive cell culture experiments with precise thermal control

The Key Features:
• Dual BioFlo 320 controllers with 10L vessels
• LAUDA chiller ensures precise temp control
• DASGIP OD4 tracks real-time biomass growth
• Exhaust gas coolers protect gas sensors
• CO₂/O₂ off-gas analysis for process insight
• Modular design fits fermentation & cell culture

Our Advantage
• We have experience servicing and repairing these instruments.
• This instrument goes through all the described service steps before shipping. see the questions section below
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Technical sales consultation available
• Remote installation and instrument operation training may be available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

Our Guarantee
• Warranty
• You get after-sales technical support
• Customer references are available
• The instrument will be fully functional!
• Smooth process from the initial quote to order fulfillment

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This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

PLEASE NOTE THAT THE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE

Q: Can I view this instrument in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.

Q: What about the warranty?
A: The instrument comes fully warranted for both parts and labor.

Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to familiarize you with this instrument.

Q: What should I watch out for when buying a used/pre-owned instrument from another lab?
A: Ensure that the instrument is in good working condition with no damage or wear that could affect its performance. Ask for details about testing steps performed, video demo, and test screenshots.

Q: Do you have more than one of these instruments on hand?
A: We generally carry multiple instruments on hand, along with a variety of spare parts.

Q: This procurement is for a University or College. Are purchase orders with NET terms accepted?
A: We accept purchase orders from all major US and Canadian universities.

Q: How fast will you ship?
A: We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.

Q: How do you pack? Have you previously packed and shipped the fragile laboratory instruments?
A: We have extensive experience shipping high-end and complex laboratory instruments in the US and Internationally.

Q: Do you ship Internationally?
A: Yes! We have shipped to multiple continents and over 30 countries.

Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new instrument, you will save 60% or more and have plenty of funds left over for an additional instrument or two.

TO VIEW ADDITIONAL PHOTOS, VIDEOS AND VERY DETAILED INFORMATION SUCH AS TEST STEPS PERFORMED, CONFIGURATION, ETC., PLEASE VISIT OUR WEB SITE. TO GET THERE PLEASE CLICK ON “WEBSTORE”; TO YOUR RIGHT AND YOU WILL SEE LINK TO OUR SITE IN THE MIDDLE OF THE SCREEN. THEN USE LAST 5 DIGITS OF MPN NUMBER IN THE SEARCH FIELD TO LOCATE YOUR ITEM.

DO NOT HESITATE TO CALL US OR EMAIL US WITH ANY QUESTIONS YOU MIGHT HAVE.

ALL OF OUR ITEMS ARE AVAILABLE FOR INSPECTION AT OUR FACILITY.

Return Policy

Items are sold as-is with no returns or refunds available unless explicitly stated.