For Sale NIKON Electrophysiology Rig Microscope

For Sale Nikon Electrophysiology Rig DIC Fluorescence Microscope w/ Sutter Micromanipulator MP-285, HEKA EPC 10 Patch Clamp, Faraday Cage

• Fully Functional
• Serviced & in Stock
• Customized to your requirements
• Includes new fluorescence filters
• Technical sales consultation available
• Continental USA 120 DAYS WARRANTY & 60 DAYS INTERNATIONAL
• Includes remote microscope training session and software overview
• More info about our Microscopy Capabilities or view the Microscope Buying Guide

Please note the whole electrophysiology setup is currently installed on the Nikon Eclipse TI-U Inverted Microscope. Should you prefer a different inverted microscope, we can swap to the following microscopes:
• Nikon: Ti-U, Current Model Nikon Ti2-U
• Zeiss: Axio Observer
• Olympus: IX70, IX71, IX73 (if available)

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As configured setup for Patch Clamp Electrophysiology with DIC studies
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Installed electrophysiology accessories
• This setup includes a Sutter MP-285 micromanipulation setup (controller, stage/holder and joystick)
• HEKA 10 USB Digital amplifier with the headstage allowing for recording using Heka Patch Master software, license dongle included
• The XY mechanical stage has a travel range of 70 x 50mm. It will allow for observation of prepared slides and petri dishes.
• Included Faraday cage is an enclosure that is used to protect the contained objects from both static and non-static electromagnetic fields
• The included TMC Anti-Vibration features TMC's Gimbal Piston Air Isolators, which allow quick dissipation of disturbances on the isolated tabletop and clear imaging without interference from local vibration around the lab.
• Enhancing components, such as Fluorescence Filter Cubes, have been added (Other components are available)
• The total current magnification range of this microscope is 40 to 200x (Other objectives are available)

Please note all the above microscopes are fully mechanical to eliminate electromagnetic interference.
Either configuration can be set up with fluorescence (DIC on Zeiss is subject to availability)
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This is what you will receive before customizing this microscope:
Standard Nikon Ti-U Microscope Configuration:

Microscopy Techniques:
Brightfield with DIC, Fluorescence Microscopy and Patch Clamp Electrophysiology

Base:
• Nikon Eclipse Ti-U Inverted Microscope
• Faraday Cage
• TMC Anti-Vibration Table
Head:
• Ti-TS B Binocular Head
• (2) CFI 10x/22 Eyepieces
Condenser:
• LWD 0.52 Condenser
• DIC NI Condenser Insert
Brightfield Path:
• TI-DH Illumination Arm
Fluorescence Path:
• TI-FL-EPI path
Fluorescence filter cube turret:
• 6-position Motorized Turret
Fluorescence Filter Cubes:
• Three empty filter cubes
• Three Brand New filter sets of either DAPI, FITC, Texas Red or CY3
*Other filters available - please inquire*
Fluorescence Illumination:
• EXFO X-Cite 120 Metal Halide w/ collimating adapter and light guide
Brightfield Illumination:
• PRIOR Brightfield LED Illumination System LDB102NI
Stage:
• Mechanical XY
• Sutter Stage attachment
Micromanipulation:
• Sutter MP-285 Micromanipulation
• Sutter ROE285-2223 Micromanipulation Stage Controller
Amplification:
• HEKA Patch Clamp EPC10 USB
Objectives:
• 6-position Mechanical Turret
• 4x/0.13 Plan Fluor
• 10x/0.30 Plan Fluor DIC L/N1
• 20x/0.75 Plan Apo VC (this is a high quality Nikon APO CFI60 Objective, it was originally installed in a custom piece of equipment where manufacturer did not want to show that they used Nikon objectives)
*Other objectives available - please inquire*
DIC Components/Polarization:
• T-A2 Analyzer
• T-P2 Polarizer
• 10x DIC prism
• 20x DIC prism
Photo/Port Splits:
• 100% Eyepiece, 0% Camera
• 20% Eyepiece, 80% Camera
Camera:
• 10MP CMOS Camera
Computer and Software:
• Computer running PatchMaster (v2x92) software w/dongle, and applicable camera software

Communication and power cables.

This configuration includes any 3 of these new filter sets.
• New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
• New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
• New filter set - TRITC/Cy3/Dsred (EX: 545/20nm, DM: 562nm, EM: 605/60nm) (Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
• New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)
• New filter set - Cy5 (May require an upgraded light source) (EX: 630/50nm, DM: 662nm, EM: 695/50nm) (Dye compatibility: Cy5/AlexaFluor 647/Draq5)

This microscope's imaging capabilities:
Imaging software highlights:
• Ability to take still images and live videos of your samples
• Display, annotate, edit, calibrate, measure, analyze, process, print, and save raster
• Variable linear measurements such as line segment, radius values, and others

We can customize this microscope to fit your requirements:
Add Microscopy Techniques (In addition to installed Brightfield, Fluorescence and DIC)
• Phase Contrast

Increase Maximum Magnification and Image Quality:
• Microscope can be configured for magnification between 40x and 1000x (with 10x Eyepieces)
• We can add standard higher magnification Nikon microscope objectives
• We can add high-end Nikon objectives

Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.
• Additional filter cubes
• Additional filter sets to match your fluorochromes.
• Xenon, Metal Halide, or LED Fluorescence microscope light sources

We will also ensure the fluorescence light source matches your selected filter sets.

Enhance imaging capabilities:
• We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.

We can configure it to work with the sample medium you need:
• Slide, Petri dish, microplate, etc

Commonly Requested Customer Upgrades
• High-End CCD, sCMOS Cameras
• LED or Metal Halide Fluorescence Light Sources
• Phase Contrast, Polarization, or the DIC configurations
• Higher Magnification Objectives
• Higher performance Fluor or APO objectives
• Motorized XY Stage (stage, controller, joystick)

We are excited to help build and configure the right microscope for you!

Nikon Ti-U Fluorescence Microscope Overview
(Final microscope capabilities will depend on the modifications you request)

The fully manual Nikon Ti-U inverted microscope offers extensive capabilities for observing samples in various biological and materials science contexts. With manual control over objectives, focus, and illumination, it provides adaptable and precise sample examination. Users can easily adjust magnification levels for detailed exploration, manipulate focus for accuracy, and customize illumination sources to enhance contrast and visibility. Its inverted design facilitates the examination of thick samples or live specimens in containers such as petri dishes or culture flasks. This microscope caters to researchers and scientists who require hands-on control and flexibility in their microscopy experiments, facilitating detailed observation and analysis across a wide range of disciplines. The addition of phase contrast on an inverted microscope allows for clear visualization and detailed examination of transparent or unstained specimens, offering enhanced contrast and revealing intricate cellular structures with remarkable clarity.

The microscope is suitable for the following applications as configured:
• Fluorescence microscopy: Ideal for fluorescent labeling and imaging of cells and tissues.
• Material science research: Suitable for examining material properties and structures.
• Biological research: Essential for cellular structure, function, and behavior studies.
• Pharmacological studies: Facilitates the investigation of drug effects on cells and tissues.
• Biotechnology applications: Enables genetic engineering and molecular biology research.
• Educational purposes: Serves as a teaching tool in universities and research institutes.

Other potential applications:
• Phase contrast and DIC: Enhances visibility of transparent specimens without staining.
• High-resolution imaging: Provides clear, detailed images for in-depth analysis of samples.

Why do so many customers choose to order from us?

Our Advantage
• We make sure to match the microscope capabilities to your requirements.
• We have experience servicing and repairing these microscopes.
• This microscope goes through an extensive set of service steps before shipping.
• We offer detailed photos and videos of the microscopes you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Many upgrade and repair parts are in stock.
• Remote installation and microscope operation training are available.
• On-site installation may be available in the Boston Area (New England area)
• Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)

Our Guarantee
• Warranty
• An extended warranty may be available.
• You get after-sales technical support.
• Customer references are available.
• The microscope will be complete and working!
• Smooth process from the initial quote to order fulfillment.

*SM* Small Crate
300#

PLEASE NOTE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE

Q: I am not 100% sure about the configuration I need, can your team assist me with selecting the right microscope for me?
A: Absolutely, we can start with your application, samples, or challenge to assist you in selecting the right configuration and the right microscope for you.

Q: Can you make the necessary modifications to the standard configuration to fit my application?
A: Certainly, we carry a large variety of upgrade components to ensure the final microscope configuration will fit your application

Q: What steps do you take to verify, service, and confirm the functionality of this Microscope?
A: Each microscope goes through extensive service to ensure it performs to specification.

Q: Can I view this microscope in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.

Q: What about the warranty?
A: The microscope comes fully warranted for both parts and labor.

Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to get you familiar with this microscope

Q: What should I watch out for when buying a used/pre-owned microscope from another lab?
A: That it has no missing, broken, or incorrect parts for your application, any mechanical or motorized components have been serviced and lubricated, controller issues, good quality filter cubes, proper and undamaged objectives, and most importantly no dust in the light path or objectives.

Q: Do you have these and other microscopes on hand?
A: We carry this and many other microscopes from Nikon, Zeiss, Olympus, and Leica.

Q: I am from the University or College, do you accept Purchase Orders?
A: We accept purchase orders from all major US and Canadian universities.

Q: How fast will you ship?
A: We can ship within 2-3 days to about 2 weeks, depending on the final configuration required.

Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.

Q: Have you packed and shipped microscopes in the past?
A: We have extensive experience in safely shipping high-end and complex microscopes all over the world.

Q: Do you ship Internationally?
A: So far, we have shipped to almost every continent and over 30 countries.

Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.

TO VIEW ADDITIONAL PHOTOS, VIDEOS AND VERY DETAILED INFORMATION SUCH AS TEST STEPS PERFORMED, CONFIGURATION, ETC., PLEASE VISIT OUR WEB SITE. TO GET THERE PLEASE CLICK ON “WEBSTORE”; TO YOUR RIGHT AND YOU WILL SEE LINK TO OUR SITE IN THE MIDDLE OF THE SCREEN. THEN USE LAST 5 DIGITS OF MPN NUMBER IN THE SEARCH FIELD TO LOCATE YOUR ITEM.

DO NOT HESITATE TO CALL US OR EMAIL US WITH ANY QUESTIONS YOU MIGHT HAVE.

ALL OF OUR ITEMS ARE AVAILABLE FOR INSPECTION AT OUR FACILITY.

Return Policy

Items are sold as-is with no returns or refunds available unless explicitly stated.