For Sale Nikon Ni-U Upright Fluorescence Microscope
• Fully Customizable
• Serviced & in Stock
• Includes new fluorescence filters
• Expert sales consultation
• Continental USA 120 DAYS WARRANTY & 60 DAYS INTERNATIONAL
• Includes remote microscope training session and software overview
• Extended warranty available
• More info about our microscopy capabilities (More info)
Predecessor to Ni-L Microscope (Ni-L has LED for brightfield instead of halogen)
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This is what you will receive before customizing this microscope:
Standard Nikon Ni-U Upright Fluorescence Microscope Configuration:
Microscopy Techniques:
Brightfield and Fluorescence Microscopy
Microscope Base:
• Nikon Eclipse Ni-U Upright Microscope
Microscope Head:
• C-TE2 Trinocular Head
• CFI 10x/22 Eyepieces (Qty. 2)
Fluorescence Light Path:
• NI-FLEI Fluorescence Light Path
• Ni-FLT6 6-position turret
• EXFO Collimating Adapter
Fluorescence Illumination:
• EXFO X-Cite 120 Metal Halide w/ light guide
Fluorescence filters:
• DAPI - EX 375/28nm DM 415nm EM 460/50nm (NEW FILTER SET)
• FITC - EX 480/35nm DM 505nm EM 535/40nm (NEW FILTER SET)
• Texas Red - EX 550/20nm DM 585nm EM 630/40nm (NEW FILTER SET)
• *Other filters available - please inquire*
Brightfield Light Path:
• Base
Brightfield Illumination:
• D-LH 100W Halogen Lamp
Objectives:
• 4x/0.13 Plan Fluor
• 10x/0.30 Plan Fluor
• 20x/0.75 Plan Apo VC (This is a high-quality Nikon APO CFI60 Objective; it was originally installed in a custom piece of equipment where manufacturer did not want to show that they used Nikon objectives)
• *Additional Objectives may be available*
Condenser:
• 1x~100x Condenser, Mechanical
Stage:
• C-CSR Mechanical XY Stage
• C-H2L Slide Adapter
Camera ports:
• Eyepieces - 100%
• Eyepieces/Camera - 50%/50%
Camera:
• Digital 10MP CMOS Camera
Computer & Software:
• Computer running applicable camera software
Accessories:
• Necessary power and communication cables
This configuration includes any 3 of these new filter sets.
• New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
• New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
• New filter set - TRITC/Cy3/Dsred (EX: 545/20nm, DM: 562nm, EM: 605/60nm) (Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
• New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)
• New filter set - Cy5 (May require an upgraded light source) (EX: 630/50nm, DM: 662nm, EM: 695/50nm) (Dye compatibility: Cy5/AlexaFluor 647/Draq5)
This microscope's imaging capabilities:
Imaging software highlights:
• Ability to take still images and live videos of your samples
• Display, annotate, edit, calibrate, measure, analyze, process, print, and save raster
• Variable linear measurements such as line segment, radius values, and others
• Open source Micromanager Multi-dimensional Image acquisition & automation software capabilities
• Multi-frame (Time lapse/Image acquisition over time)
We can customize this microscope to fit your requirements:
Add Microscopy Techniques (In addition to installed Brightfield and Fluorescence)
• Phase Contrast, DIC, or Polarization Add-on Components may be added (subject to availability)
Increase Maximum Magnification and Image Quality:
• Microscope can be configured for magnification between 40x and 200x (with 10x Eyepieces)
• We can add standard higher magnification Nikon microscope objectives
• We can add high-end Nikon Apochromat objectives
Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.
• Additional filter cubes
• Additional filter sets to match your fluorochromes.
• Xenon or LED Fluorescence microscope light sources
We will also ensure the fluorescence light source matches your selected filter sets.
Enhance imaging capabilities:
• We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.
Add other Motorized/Automation Capabilities:
• Motorized high-speed shutter
• Motorized filter cube turret
• Motorized objective(nosepiece) turret
We can configure it to work with the sample medium you need:
• Slide, Petri dish, microplate, etc.
Commonly Requested Customer Upgrades
• High-End CCD, sCMOS Cameras
• LED or Metal Halide Fluorescence Light Sources
• Phase Contrast, Polarization, or the DIC configurations
• Higher Magnification Objectives
• Higher performance Fluor or APO objectives
• Motorized components (controller, joystick)
We are excited to help build and configure the right microscope for you!
Nikon Ni-U Upright Fluorescence Microscope Overview
(Final microscope capabilities will depend on the modifications you request)
• This microscope can be used in biological studies.
• Enhancing components, such as Fluorescence Filter Cubes have been added.
• The total current magnification range of this microscope with 10x eyepieces is 40x to 200x.
• This microscope is compatible with slides. (Options for petri dishes and microplates may be available).
• The fluorescence light source included is a EXFO X-Cite 120 Metal Halide.
• Ergonomic tube and stage height adjustment mechanism of the microscope allow comfortable viewing positions.
Suited for the following applications:
• Fluorescence Microscopy: Visualization of fluorescently labeled specimens, such as cells and tissues.
• Brightfield Microscopy: General observation and analysis of stained samples.
Other potential applications:
• Phase Contrast Microscopy: Enhancing the contrast of transparent specimens.
• DIC (Differential Interference Contrast) Microscopy: Providing high-contrast images of unstained specimens.
• Darkfield Microscopy: Observing live, unstained specimens with high contrast.
• Confocal Microscopy (with appropriate attachments): High-resolution, 3D imaging of fluorescently labeled specimens.
• Live Cell Imaging: Studying live cells and their dynamic processes over time.
Why should you buy from us?
Our Advantage
• We make sure to match the microscope capabilities to your requirements.
• We have experience servicing and repairing these microscopes.
• This microscope goes through an extensive set of service steps before shipping.
• We offer detailed photos and videos of the microscopes you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Many upgrade and repair parts are in stock.
• Remote installation and microscope operation training are available.
• On-site installation may be available in the Boston Area (New England area)
• Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)
Our Guarantee
• It will work!
• Continental US Warranty 120 Days & 60 Days International.
• An extended warranty may be available.
• You get after-sales technical support.
• Smooth process from the initial quote to order fulfillment.
• Customer references are available.
This will ship via freight. Please contact us with your zip code to obtain shipping quote.
*SM* Small Crate
300#
PLEASE NOTE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE
Q: I am not 100% sure about the configuration I need, can your team assist me with selecting the right microscope for me?
A: Absolutely, we can start with your application, samples, or challenge to assist you in selecting the right configuration and the right microscope for you.
Q: Can you make the necessary modifications to the standard configuration to fit my application?
A: Certainly, we carry a large variety of upgrade components to ensure the final microscope configuration will fit your application
Q: What steps do you take to verify, service, and confirm the functionality of this Microscope?
A: Each microscope goes through extensive service to ensure it performs to specification.
Q: Can I view this microscope in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.
Q: What about the warranty?
A: The microscope comes fully warranted for both parts and labor.
Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to get you familiar with this microscope
Q: What should I watch out for when buying a used/pre-owned microscope from another lab?
A: That it has no missing, broken, or incorrect parts for your application, any mechanical or motorized components have been serviced and lubricated, controller issues, good quality filter cubes, proper and undamaged objectives, and most importantly no dust in the light path or objectives.
Q: Do you have these and other microscopes on hand?
A: We carry this and many other microscopes from Nikon, Zeiss, Olympus, and Leica.
Q: I am from the University or College, do you accept Purchase Orders?
A: We accept purchase orders from all major US and Canadian universities.
Q: How fast will you ship?
A: We can ship within 2-3 days to about 2 weeks, depending on the final configuration required.
Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.
Q: Have you packed and shipped microscopes in the past?
A: We have extensive experience in safely shipping high-end and complex microscopes all over the world.
Q: Do you ship Internationally?
A: So far, we have shipped to almost every continent and over 30 countries.
Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.
TO VIEW ADDITIONAL PHOTOS, VIDEOS AND VERY DETAILED INFORMATION SUCH AS TEST STEPS PERFORMED, CONFIGURATION, ETC., PLEASE VISIT OUR WEB SITE. TO GET THERE PLEASE CLICK ON “WEBSTORE”; TO YOUR RIGHT AND YOU WILL SEE LINK TO OUR SITE IN THE MIDDLE OF THE SCREEN. THEN USE LAST 5 DIGITS OF MPN NUMBER IN THE SEARCH FIELD TO LOCATE YOUR ITEM.
DO NOT HESITATE TO CALL US OR EMAIL US WITH ANY QUESTIONS YOU MIGHT HAVE.
ALL OF OUR ITEMS ARE AVAILABLE FOR INSPECTION AT OUR FACILITY.
Return Policy
Items are sold as-is with no returns or refunds available unless explicitly stated.