For Sale NIKON Ti2-E PFS Microscope

For Sale Nikon Ti2-E PFS N-SIM Super-Resolution Inverted Motorized Microscope with Dual Fluorescence Illumination

• Fully Functional
• Serviced & in Stock
• Customized to your requirements
• Includes new fluorescence filters
• Technical sales consultation available
• Continental USA 180 DAYS WARRANTY & 60 DAYS INTERNATIONAL
• Includes remote microscope training session and software overview

Service Highlights
• Optics quality is checked for field distortion and uniformity at the specimen plane
• Fluorescence Intensity is also measured at the specimen plane to confirm light source and filter performance
• Preventive Maintenance (PM) is Completed

More info about our Microscopy Capabilities or view the Microscope Buying Guide
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Please note The N-SIM module is not currently configured and will require a visit from a Nikon technician to set up. Microscope itself is fully functional and was tested.

This is what you will receive before customizing this microscope:
Standard Nikon Ti2-E Inverted Microscope Configuration:
Microscopy Techniques:
Brightfield and Fluorescence Microscopy
Microscope Base:
• Nikon Ti2-E Inverted Microscope
• TI2-CTRE Controller
Microscope Head:
• TC-T-TS Binocular Head
• (2) CFI 10x/22 Eyepieces
Dual Fluorescence Illumination Paths: (Laser + Widefield)
• N-SIM E illuminator unit
• TI2-LA-BF fluorescence light path w/ TI2-LA-FL light guide adapter
Fluorescence Illumination:
• Nikon LU-N3-SIM laser illumination unit (structured illumination / laser-based system)
• Lumencor Sola Reflective Light Source (widefield fluorescence)
Fluorescence filter cube turret:
• (2) TI2-F-FLT-E Motorized 6-position filter cube turrets
Fluorescence filters:
• Quad SIM 405/488/561/640
• HM Half Mirror
• Three Brand New filter sets of either DAPI, FITC, Texas Red or CY3
• Other filters available - please inquire
Emission Filter Wheel:
• TI2-P-FWB-E motorized BA filter wheel
• Installed filters: EM525/50, EM605/70, EM700/75
Brightfield Light Path:
• TI2-D-PD Transmitted Illumination Pillar
Brightfield Illumination:
• TI2-D-LHLED LED Brightfield Light Source
Objective Turret:
• 6-Position Motorized Objective turret
• Perfect Focus System (PFS)
Objectives:
• Nikon Plan Apo λ 10x/0.45
• Nikon S Fluor 20x/0.75
• Nikon Apo 60x/1.40 Oil λS
Condenser:
• TC-C-TC 7-position Mechanical Condenser
• LWD 0.52 Condenser Lens
Stage:
• TI2-S-SE-E Motorized Stage
• MCL Nano-Drive piezo stage controller
• PI E-753 digital piezo controller
• Nikon TI2-S-JS joystick controller
Camera ports:
• 100% Eyes
• 100% Left
• 100% Right
• 50% Eyes / 50% Left
Camera:
• Hamamatsu ORCA-Flash4.0 Monochrome sCMOS Camera
Computer & Software:
• Desktop computer running NIS-Elements AR software with a license dongle.
Accessories:
• Environmental enclosure (draft shield for thermal and airflow stability) (No active temperature or CO₂ control installed)
• Necessary power and communication cables

Please note The N-SIM module is not currently configured and will require a visit from a Nikon technician to set up. Microscope itself is fully functional and was tested.

This configuration includes any 3 of these new filter sets.
• New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
• New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
• New filter set - TRITC/Cy3/Dsred (EX: 545/20nm, DM: 562nm, EM: 605/60nm) (Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
• New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)
• New filter set - Cy5 (May require an upgraded light source) (EX: 630/50nm, DM: 662nm, EM: 695/50nm) (Dye compatibility: Cy5/AlexaFluor 647/Draq5)

Automated/motorized capabilities of this microscope:
Installed automation/motorized components:
• Motorized fluorescence imaging with automated filter switching
• Automated multi-position XY stage scanning (tiling, plate imaging)
• Motorized objective switching via 6-position turret
• Motorized emission filter wheel for rapid channel selection
• Automated fluorescence cube switching (dual turrets)
• Motorized focus drive with Perfect Focus System (PFS) for drift correction
• High-speed Z positioning via piezo nanopositioning (fast Z-stack / SIM-ready)
• Integrated joystick control for manual + assisted navigation
• Computer-controlled acquisition via NIS-Elements (multi-dimensional imaging)
• Dual illumination automation (laser + LED fluorescence switching)

This microscope's imaging capabilities:
• Brightfield and multi-channel fluorescence imaging
• Structured illumination (SIM-capable hardware configuration)
• High-resolution imaging with Plan Apo and oil immersion objectives
• Fast Z-stack acquisition with piezo-driven nanopositioning
• Long-term live imaging with Perfect Focus System (PFS)
• Large-area tile scanning and multi-position imaging via motorized stage

Imaging software highlights:
• NIS-Elements AR for multi-dimensional acquisition (XY, Z, time, channels)
• Automated stage mapping and tile scan stitching
• Hardware synchronization with illumination and filter switching
• Real-time image acquisition and device control
• Support for multi-channel fluorescence workflows
• Preset-based experiment automation and reproducibility

We can customize this microscope to fit your requirements:
Add Microscopy Techniques (In addition to installed Brightfield and Fluorescence)
• Phase Contrast, DIC, or Polarization Add-on Components may be added (subject to availability)

Increase Maximum Magnification and Image Quality:
• Microscope can be configured for magnification between 100x and 600x (with 10x Eyepieces)
• We can add standard higher magnification Nikon microscope objectives
• We can add high-end Nikon Apochromat objectives

Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.
• Additional filter cubes
• Additional filter sets to match your fluorochromes.
• Xenon, Metal Halide, or LED Fluorescence microscope light sources

We will also ensure the fluorescence light source matches your selected filter sets.

Enhance imaging capabilities:
• We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.

We can configure it to work with the sample medium you need:
• Slide, Petri dish, microplate, etc.

Commonly Requested Customer Upgrades
• High-End CCD, sCMOS Cameras
• Phase Contrast, Polarization, or the DIC configurations
• Higher Magnification Objectives
• Higher performance Fluor or APO objectives

We are excited to help build and configure the right microscope for you!

Nikon Ti2-E Inverted Microscope Overview
(Final microscope capabilities will depend on the modifications you request)
• Motorized Ti2-E inverted microscope with controller and precision XY stage for automated positioning and imaging.
• Dual fluorescence illumination combining laser-based and LED light sources for flexible multi-channel imaging.
• Motorized filter turrets and emission filter wheel enable rapid, automated fluorescence channel switching.
• Motorized objective turret with high-performance optics supports high-resolution and multi-magnification imaging.
• Integrated Perfect Focus System maintains stable focus during long or multi-position experiments.
• High-sensitivity sCMOS camera, transmitted LED illumination, and environmental enclosure support stable imaging conditions.

Suited for the following applications as configured:
• Multi-channel fluorescence imaging and quantitative analysis
• High-resolution imaging with oil and high-NA objectives
• Automated tile scanning and large-area sample mapping
• Time-lapse imaging with focus stabilization (PFS)
• Fast Z-stack acquisition using motorized and piezo control
• Structured illumination (SIM-ready) imaging workflows

Other potential applications:
• Routine brightfield imaging and sample documentation
• Fixed-cell fluorescence and immunostaining analysis
• Live-cell imaging with environmental control integration
• High-content screening and plate-based assays
• Microfluidics and perfusion-based experiments
• Materials and thin-film inspection under transmitted or fluorescence light

REASONS WHY THESE CUSTOMERS CHOOSE TO ORDER FROM US

• They want to ensure the microscope is customized to fit their application
• They want to ensure that internal optics are properly cleaned
• They want to ensure that internal mechanical and motorized mechanisms are lubricated and aligned
• They want to ensure that image quality is verified at the sample point for field distortion and uniformity
• They want to ensure that the fluorescence intensity is verified at the sample point
• They want to ensure NEW fluorescence filters are installed just the same as buying a new microscope
• They want to ensure that if paired with a motorized XY stage, it is lubricated and does not skip steps
• They want an option to upgrade the camera to sCMOS and the light source to Lumencor or X-Cite LED
• They want training to learn how to operate the microscope
• They want a reliable warranty
• They want peace of mind knowing it will be safely and professionally packed for shipping

Our Advantage
• We make sure to match the microscope capabilities to your requirements.
• We have experience servicing and repairing these microscopes.
• This microscope goes through an extensive set of service steps before shipping.
• We offer detailed photos and videos of the microscopes you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Many upgrade and repair parts are in stock.
• Remote installation and microscope operation training are available.
• On-site installation may be available in the Boston Area (New England area)
• Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)

Our Guarantee
• Warranty
• An extended warranty may be available.
• You get after-sales technical support.
• Customer references are available.
• The microscope will be complete and working!
• Smooth process from the initial quote to order fulfillment.

*SM* Small Crate
300#

PLEASE NOTE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE

Q: I am not 100% sure about the configuration I need, can your team assist me with selecting the right microscope for me?
A: Absolutely, we can start with your application, samples, or challenge to assist you in selecting the right configuration and the right microscope for you.

Q: Can you make the necessary modifications to the standard configuration to fit my application?
A: Certainly, we carry a large variety of upgrade components to ensure the final microscope configuration will fit your application

Q: What steps do you take to verify, service, and confirm the functionality of this Microscope?
A: Each microscope goes through extensive service to ensure it performs to specification.

Q: Can I view this microscope in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.

Q: What about the warranty?
A: The microscope comes fully warranted for both parts and labor.

Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to get you familiar with this microscope

Q: What should I watch out for when buying a used/pre-owned microscope from another lab?
A: That it has no missing, broken, or incorrect parts for your application, any mechanical or motorized components have been serviced and lubricated, controller issues, good quality filter cubes, proper and undamaged objectives, and most importantly no dust in the light path or objectives.

Q: Do you have these and other microscopes on hand?
A: We carry this and many other microscopes from Nikon, Zeiss, Olympus, and Leica.

Q: I am from the University or College, do you accept Purchase Orders?
A: We accept purchase orders from all major US and Canadian universities.

Q: How fast will you ship?
A: We can ship within 2-3 days to about 2 weeks, depending on the final configuration required.

Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.

Q: Have you packed and shipped microscopes in the past?
A: We have extensive experience in safely shipping high-end and complex microscopes all over the world.

Q: Do you ship Internationally?
A: So far, we have shipped to almost every continent and over 30 countries.

Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.

TO VIEW ADDITIONAL PHOTOS, VIDEOS AND VERY DETAILED INFORMATION SUCH AS TEST STEPS PERFORMED, CONFIGURATION, ETC., PLEASE VISIT OUR WEB SITE. TO GET THERE PLEASE CLICK ON “WEBSTORE”; TO YOUR RIGHT AND YOU WILL SEE LINK TO OUR SITE IN THE MIDDLE OF THE SCREEN. THEN USE LAST 5 DIGITS OF MPN NUMBER IN THE SEARCH FIELD TO LOCATE YOUR ITEM.

DO NOT HESITATE TO CALL US OR EMAIL US WITH ANY QUESTIONS YOU MIGHT HAVE.

ALL OF OUR ITEMS ARE AVAILABLE FOR INSPECTION AT OUR FACILITY.

Return Policy

Items are sold as-is with no returns or refunds available unless explicitly stated.