For Sale NIKON Ti2-E Microscope

For Sale Nikon Eclipse Ti2-E PFS Inverted LED Fluorescence DIC Motorized Microscope

CURRENTLY SOLD OUT, IF YOU ARE INTERESTED PLEASE SUBMIT YOUR INTEREST AND WE WILL UPDATE YOU ONCE INSTRUMENT IS BACK IN STOCK.

• Fully Functional
• Serviced & in Stock
• Customized to your requirements
• Includes new fluorescence filters
• Technical sales consultation available
• Continental USA 180 DAYS WARRANTY & 60 DAYS INTERNATIONAL
• Includes remote microscope training session and software overview

Service Highlights
• Optics quality is checked for field distortion and uniformity at the specimen plane
• Fluorescence Intensity is also measured at the specimen plane to confirm light source and filter performance
• Preventive Maintenance (PM) is Completed

More info about our Microscopy Capabilities or view the Microscope Buying Guide
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This is what you will receive before customizing this microscope:
Standard Nikon Eclipse Ti2-E PFS (Perfect Focus system) Microscope Configuration:

Microscopy Techniques:
Brightfield and Fluorescence & DIC Microscopy

Microscope Base:
• Nikon Ti2-E PFS Inverted Microscope
• Nikon TI2-CTRE Control Box
Microscope Head:
• TC-T-TS Binocular Head
• CFI 10x/22 Eyepiece (Qty. 2)
Fluorescence Light Path:
• Liquid Light Guide
• Reflective Light Path
Fluorescence Illumination:
• Lumencor LED Multicolor Spectra Light Engine
Fluorescence filter cube turret:
• Fluorescence Cube Motorized 6-position turret TI2-F-FLT-E
Fluorescence filters:
• Three empty filter cubes
• Three Brand New filter sets of either DAPI, FITC, Texas Red or CY3
• *Other filters available - please inquire*
Brightfield Illumination:
• Pillar for transmitted illumination TI2-D-PD
• LED Lamphouse for dia illumination TI2-D-LHLED
Objective Turret:
• Perfect Focus Unit with motorized 6-position nosepiece (Ti2-N-ND-P)
Objectives:
• 4x Plan Fluor
• 10x/0.30 Plan Fluor
• 20x/0.75 Plan APO VC DIC
Condenser:
• Mechanical Condenser Turret
• LWD Manual Condenser Lens with 0.52 Lens
• T-P2 Polarizer
• T-A2 Analyzer
• One DIC Condenser & Objective Insert
Stage:
• Nikon TI2-CTRE Control Box
• ProScan III H31XYZE Microscope Controller
• Prior ProScan H117P1N4/F Motorized Stage
• PRIOR CS200XY Joystick
Photo/Port Splits:
• Left - 100%
• Right - 100%
• Eyepieces - 100%
• Eyepieces/Left - 20%/80%
Camera:
• Bestscope 8.3MP color CMOS camera
Computer & Software:
• Computer running applicable software
Accessories:
• Necessary power and communication cables

This configuration includes any 3 of these new filter sets.
• New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
• New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
• New filter set - TRITC/Cy3/Dsred (EX: 545/20nm, DM: 562nm, EM: 605/60nm) (Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
• New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)
• New filter set - Cy5 (May require an upgraded light source) (EX: 630/50nm, DM: 662nm, EM: 695/50nm) (Dye compatibility: Cy5/AlexaFluor 647/Draq5)

Automated/motorized capabilities of this microscope:
Installed automation/motorized components:
• Motorized XY Stage
• Motorized Objective Nosepiece
• Motorized Fluorescence Filter Turret
• Motorized Z axis with drift control PFS

This microscope's imaging capabilities:
Imaging software highlights:
• Ability to take still images and live videos of your samples
• Display, annotate, edit, calibrate, measure, analyze, process, print, and save raster
• Variable linear measurements such as line segment, radius values, and others
• Open source Micromanager Multi-dimensional Image acquisition & automation software capabilities
• Multi-frame (Time lapse/Image acquisition over time)
• *Multi-position (XY coordinate/Stitching/Mosaic) – only available when equipped with a motorized XY Stage

We can customize this microscope to fit your requirements:
Add Microscopy Techniques (In addition to installed Brightfield, DIC and Fluorescence)
• Phase Contrast, or Polarization Add-on Components may be added (subject to availability)

Increase Maximum Magnification and Image Quality:
• Microscope can be configured for magnification between 40x and 200x (with 10x Eyepieces)
• We can add standard higher magnification Nikon microscope objectives
• We can add high-end Nikon Apochromat objectives

Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.
• Additional filter cubes
• Additional filter sets to match your fluorochromes.
• Xenon, Metal Halide, or LED Fluorescence microscope light sources

We will also ensure the fluorescence light source matches your selected filter sets.

Enhance imaging capabilities:
• We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.

We can configure it to work with the sample medium you need:
• Slide, Petri dish, microplate, etc.

Commonly Requested Customer Upgrades
• High-End CCD, sCMOS Cameras
• LED or Metal Halide Fluorescence Light Sources
• Phase Contrast, Polarization configurations
• Higher Magnification Objectives
• Higher performance Fluor or APO objectives

We are excited to help build and configure the right microscope for you!

Nikon Eclipse Ti2-E PFS Microscope Overview
(Final microscope capabilities will depend on the modifications you request)
• Inverted research microscope platform designed for advanced life science imaging and system customization
• Supports brightfield, fluorescence, and DIC microscopy with motorized optical and mechanical components
• High-stability Ti2-E base with precision control for illumination, focus, objectives, and stage movement
• Motorized fluorescence filter turret and LED light engine enable flexible multi-channel fluorescence imaging
• Motorized objective turret and precision condenser support high-quality contrast and resolution
• Expandable modular architecture allows integration of advanced accessories, cameras, and automation systems

Suited for the following applications as configured:
• Routine brightfield imaging of fixed and live cell cultures
• Multichannel fluorescence imaging using DAPI, FITC, TRITC/Texas Red, or Cy3 filter sets
• Differential Interference Contrast (DIC) imaging for enhanced contrast of unstained specimens
• Automated multi-position imaging using a motorized stage and objective turret
• Documentation and analysis of samples using a high-resolution color CMOS camera
• Time-lapse microscopy with automated focus and stage positioning

PFS System overview
• Automatic focus stability that maintains precise focus over long imaging sessions
• Real-time drift correction that compensates for temperature and mechanical changes
• Near-infrared focus tracking and no interference with visible or fluorescence imaging
• Ideal for time-lapse imaging and ensures consistent results across hours or days
• Non-invasive to samples and no added photobleaching or phototoxicity

Other potential applications:
• Live-cell imaging with environmental control add-ons
• High-content screening with additional automation and analysis hardware
• Quantitative fluorescence measurements with calibrated detectors and filters
• Advanced multi-color fluorescence imaging with expanded filter sets
• Integration with microfluidic or perfusion systems for dynamic experiments

REASONS WHY THESE CUSTOMERS CHOOSE TO ORDER FROM US
• They want to ensure the microscope is customized to fit their application
• They want to ensure that internal optics are properly cleaned
• They want to ensure that internal mechanical and motorized mechanisms are lubricated and aligned
• They want to ensure that image quality is verified at the sample point for field distortion and uniformity
• They want to ensure that the fluorescence intensity is verified at the sample point
• They want to ensure NEW fluorescence filters are installed just the same as buying a new microscope
• They want to ensure that if paired with a motorized XY stage, it is lubricated and does not skip steps
• They want an option to upgrade the camera to sCMOS and the light source to Lumencor or X-Cite LED
• They want training to learn how to operate the microscope
• They want a reliable warranty
• They want peace of mind knowing it will be safely and professionally packed for shipping

Our Advantage
• We make sure to match the microscope capabilities to your requirements.
• We have experience servicing and repairing these microscopes.
• This microscope goes through an extensive set of service steps before shipping.
• We offer detailed photos and videos of the microscopes you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Many upgrade and repair parts are in stock.
• Remote installation and microscope operation training are available.
• On-site installation may be available in the Boston Area (New England area)
• Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)

Our Guarantee
• Warranty
• An extended warranty may be available.
• You get after-sales technical support.
• Customer references are available.
• The microscope will be complete and working!
• Smooth process from the initial quote to order fulfillment.

*SM* Small Crate
300#

PLEASE NOTE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE

Q: I am not 100% sure about the configuration I need, can your team assist me with selecting the right microscope for me?
A: Absolutely, we can start with your application, samples, or challenge to assist you in selecting the right configuration and the right microscope for you.

Q: Can you make the necessary modifications to the standard configuration to fit my application?
A: Certainly, we carry a large variety of upgrade components to ensure the final microscope configuration will fit your application

Q: What steps do you take to verify, service, and confirm the functionality of this Microscope?
A: Each microscope goes through extensive service to ensure it performs to specification.

Q: Can I view this microscope in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.

Q: What about the warranty?
A: The microscope comes fully warranted for both parts and labor.

Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to get you familiar with this microscope

Q: What should I watch out for when buying a used/pre-owned microscope from another lab?
A: That it has no missing, broken, or incorrect parts for your application, any mechanical or motorized components have been serviced and lubricated, controller issues, good quality filter cubes, proper and undamaged objectives, and most importantly no dust in the light path or objectives.

Q: Do you have these and other microscopes on hand?
A: We carry this and many other microscopes from Nikon, Zeiss, Olympus, and Leica.

Q: I am from the University or College, do you accept Purchase Orders?
A: We accept purchase orders from all major US and Canadian universities.

Q: How fast will you ship?
A: We can ship within 2-3 days to about 2 weeks, depending on the final configuration required.

Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.

Q: Have you packed and shipped microscopes in the past?
A: We have extensive experience in safely shipping high-end and complex microscopes all over the world.

Q: Do you ship Internationally?
A: So far, we have shipped to almost every continent and over 30 countries.

Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.

TO VIEW ADDITIONAL PHOTOS, VIDEOS AND VERY DETAILED INFORMATION SUCH AS TEST STEPS PERFORMED, CONFIGURATION, ETC., PLEASE VISIT OUR WEB SITE. TO GET THERE PLEASE CLICK ON “WEBSTORE”; TO YOUR RIGHT AND YOU WILL SEE LINK TO OUR SITE IN THE MIDDLE OF THE SCREEN. THEN USE LAST 5 DIGITS OF MPN NUMBER IN THE SEARCH FIELD TO LOCATE YOUR ITEM.

DO NOT HESITATE TO CALL US OR EMAIL US WITH ANY QUESTIONS YOU MIGHT HAVE.

ALL OF OUR ITEMS ARE AVAILABLE FOR INSPECTION AT OUR FACILITY.

Return Policy

Items are sold as-is with no returns or refunds available unless explicitly stated.