For Sale Nikon Ti2-U Inverted Fluorescence Microscope
• Fully Functional
• Serviced & in Stock
• Customized to your requirements
• Includes new fluorescence filters
• Technical sales consultation available
• Continental USA 120 DAYS WARRANTY & 60 DAYS INTERNATIONAL
• Includes remote microscope training session and software overview
• More info about our Microscopy Capabilities or view the Microscope Buying Guide
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This is what you will receive before customizing this microscope:
Standard Nikon Ti2-U Inverted Microscope Configuration:
Microscopy Techniques:
Brightfield transmitted with Reflective Fluorescence Microscopy
Microscope Base:
• Nikon Ti2-U Inverted Microscope
Microscope Head:
• Binocular Head
• (2) CFI 10x/22 Eyepieces
Fluorescence Light Path:
• Fluorescence Light Path
• TI2-LA-FLL Collimating Adapter
Fluorescence Illumination:
• EXFO X-Cite 120PC-Q Metal Halide Fluorescence Light Source
Fluorescence filter cube turret:
• TI2-F-FLT-I 6-position Motorized Turret
Fluorescence filters:
• Three empty filter cubes
• Three Brand New filter sets of either DAPI, FITC, Texas Red or CY3
• *Other filter cubes available - please inquire*
Brightfield Light Path:
• TI2-D-PD Illumination Arm
Brightfield Illumination:
• D-LH/LC 12V 100W Halogen Lamp
Objective Turret:
• Ti2-N-N 6-position Mechanical Turret
Objectives:
• 4x/0.13 Plan Fluor
• 10x/0.30 Plan Fluor
• 20x/0.75 Plan Apo VC (this is a high-quality Nikon APO CFI60 Objective, it was originally installed in a custom piece of equipment where manufacturer did not want to show that they used Nikon objectives)
• *Other objectives available - please inquire*
Condenser:
• TC-C 5-position Mechanical Condenser Turret
• LWD 0.52 Condenser Lens
Stage:
• Mechanical XY stage
Camera ports:
• Left 100%
• Right 100%
• Eyepiece 100%
• AUX 100%
Camera:
• BestScope 830EC Digital Camera
Computer & Software:
• Computer running applicable camera software
Accessories:
• Necessary power and communication cables
This configuration includes any 3 of these new filter sets.
• New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
• New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
• New filter set - TRITC/Cy3/Dsred (EX: 545/20nm, DM: 562nm, EM: 605/60nm) (Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
• New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)
• New filter set - Cy5 (May require an upgraded light source) (EX: 630/50nm, DM: 662nm, EM: 695/50nm) (Dye compatibility: Cy5/AlexaFluor 647/Draq5)
Automated/motorized capabilities of this microscope:
Installed automation/motorized components:
• Motorized filter cube turret
This microscope's imaging capabilities:
Imaging software highlights:
• Ability to take still images and live videos of your samples
• Display, annotate, edit, calibrate, measure, analyze, process, print, and save raster
• Variable linear measurements such as line segment, radius values, and others
• Open source Micromanager Multi-dimensional Image acquisition & automation software capabilities
• Multi-frame (Time lapse/Image acquisition over time)
We can customize this microscope to fit your requirements:
Add Microscopy Techniques (In addition to installed Brightfield and Fluorescence)
• Phase Contrast, DIC, or Polarization Add-on Components may be added (subject to availability)
Increase Maximum Magnification and Image Quality:
• Microscope can be configured for magnification between 40x and 300x (with 10x Eyepieces)
• We can add standard higher magnification Nikon microscope objectives
• We can add high-end Nikon Apochromat objectives
Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.
• Additional filter cubes
• Additional filter sets to match your fluorochromes.
• Xenon, Metal Halide, or LED Fluorescence microscope light sources
We will also ensure the fluorescence light source matches your selected filter sets.
Enhance imaging capabilities:
• We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.
Add other Motorized/Automation Capabilities:
In addition to installed motorized capabilities, the microscope can be configured
• Motorized stage
• Motorized lightpath
• Motorized/automated Z-Axis Drive
• Motorized objective(nosepiece) turret
• Motorized condenser turret
We can configure it to work with the sample medium you need:
• Slide, Petri dish, microplate, etc.
• It may also be set up with a motorized XY stage for multi-slide or full microplate scans.
Commonly Requested Customer Upgrades
• High-End CCD, sCMOS Cameras
• LED or Metal Halide Fluorescence Light Sources
• Phase Contrast, Polarization, or the DIC configurations
• Higher Magnification Objectives
• Higher performance Fluor or APO objectives
• Motorized XY Stage (stage, controller, joystick)
We are excited to help build and configure the right microscope for you!
Nikon Ti2-U Inverted Microscope Overview:
(Final microscope capabilities will depend on the modifications you request)
• This Nikon Ti2-U Inverted Fluorescence Microscope is currently set up for Brightfield transmitted with Reflective Fluorescence studies.
• This microscope, with its 10x eyepieces and objectives of 4x, 10x, and 20x and an internal magnification of 1x-1.5x can has a total range of 40x to 300x magnification.
• The BestScope 830 digital color camera is capable of taking detailed digital images.
• This microscope is compatible with slides and petri dishes. (Options for microplates may be available).
• The system includes a laptop computer running the applicable software for live feed and still images.
Suited for the following applications as configured:
• Fixed cell imaging using DAPI, FITC, and Texas Red/Cy3 fluorescence channels
• Basic live-cell observation under brightfield with 4x–20x magnification
• Immunofluorescence assays with high-quality Plan Apo VC 20x objective
• Tissue section analysis using brightfield transmitted light
• General cell morphology and tracking in culture dishes or chambered slides
• Multi-channel fluorescence documentation with motorized filter turret and digital camera
Other potential applications:
• Live-cell time-lapse imaging with motorized stage, Z-drive, and incubation chamber
• High-content screening in multi-well plates with motorized XY stage and automation
• 3D Z-stack imaging using motorized focus drive and extended depth objectives
• Co-localization and multi-label fluorescence with expanded filter sets (e.g., Cy5, mCherry)
• FRET or FRAP studies using dedicated filter sets and fast switching illumination
• Quantitative fluorescence analysis with high-sensitivity sCMOS camera upgrade
Why do so many customers choose to order from us?
Our Advantage
• We make sure to match the microscope capabilities to your requirements.
• We have experience servicing and repairing these microscopes.
• This microscope goes through an extensive set of service steps before shipping.
• We offer detailed photos and videos of the microscopes you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Many upgrade and repair parts are in stock.
• Remote installation and microscope operation training are available.
• On-site installation may be available in the Boston Area (New England area)
• Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)
Our Guarantee
• Warranty
• An extended warranty may be available.
• You get after-sales technical support.
• Customer references are available.
• The microscope will be complete and working!
• Smooth process from the initial quote to order fulfillment.
*SM* Small Crate
260#
PLEASE NOTE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE
Q: I am not 100% sure about the configuration I need, can your team assist me with selecting the right microscope for me?
A: Absolutely, we can start with your application, samples, or challenge to assist you in selecting the right configuration and the right microscope for you.
Q: Can you make the necessary modifications to the standard configuration to fit my application?
A: Certainly, we carry a large variety of upgrade components to ensure the final microscope configuration will fit your application
Q: What steps do you take to verify, service, and confirm the functionality of this Microscope?
A: Each microscope goes through extensive service to ensure it performs to specification.
Q: Can I view this microscope in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.
Q: What about the warranty?
A: The microscope comes fully warranted for both parts and labor.
Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to get you familiar with this microscope
Q: What should I watch out for when buying a used/pre-owned microscope from another lab?
A: That it has no missing, broken, or incorrect parts for your application, any mechanical or motorized components have been serviced and lubricated, controller issues, good quality filter cubes, proper and undamaged objectives, and most importantly no dust in the light path or objectives.
Q: Do you have these and other microscopes on hand?
A: We carry this and many other microscopes from Nikon, Zeiss, Olympus, and Leica.
Q: I am from the University or College, do you accept Purchase Orders?
A: We accept purchase orders from all major US and Canadian universities.
Q: How fast will you ship?
A: We can ship within 2-3 days to about 2 weeks, depending on the final configuration required.
Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.
Q: Have you packed and shipped microscopes in the past?
A: We have extensive experience in safely shipping high-end and complex microscopes all over the world.
Q: Do you ship Internationally?
A: So far, we have shipped to almost every continent and over 30 countries.
Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.
TO VIEW ADDITIONAL PHOTOS, VIDEOS AND VERY DETAILED INFORMATION SUCH AS TEST STEPS PERFORMED, CONFIGURATION, ETC., PLEASE VISIT OUR WEB SITE. TO GET THERE PLEASE CLICK ON “WEBSTORE”; TO YOUR RIGHT AND YOU WILL SEE LINK TO OUR SITE IN THE MIDDLE OF THE SCREEN. THEN USE LAST 5 DIGITS OF MPN NUMBER IN THE SEARCH FIELD TO LOCATE YOUR ITEM.
DO NOT HESITATE TO CALL US OR EMAIL US WITH ANY QUESTIONS YOU MIGHT HAVE.
ALL OF OUR ITEMS ARE AVAILABLE FOR INSPECTION AT OUR FACILITY.
Return Policy
Items are sold as-is with no returns or refunds available unless explicitly stated.