CHALFONT ST GILES, UK - GE Healthcare announced last week the availability of ECL Plex, a detection system that will enable researchers to perform simultaneous multi-wavelength protein expression analysis on Western blots using Cy3 and Cy5 fluorescent dye labels. The new system is designed for existing multipurpose imagers and does not require the user to purchase additional capital equipment.

Western blotting is an important tool in protein analysis and GE Healthcare already provides leading products based on the detection of chemiluminescent or chemifluorescent signals including ECL, ECL Plus and ECL Advance Western blotting systems. The new ECL Plex system complements these products by providing customers with increased reproducibility and sensitivity when quantifying levels of protein expression using simple direct fluorescence detection without the need for enzyme substrate amplification.

The ECL Plex system consists of products selected and optimized for best performance regarding sensitivity, dynamic range, linearity, and signal-to-noise ratio. In the multiplex application, two proteins can be detected with minimal cross-reactivity between antibodies and the long-lasting signal allows stored blots to be rescanned months later.
The optimized ECL Plex system is ideally suited for use with the Typhoon imager and can also be used with Ettan DIGE and other multi-purpose imagers. ECL Plex is fully compatible with Hybond ECL (nitrocellulose) and the new Hybond-LFP (low-fluorescent PVDF) membrane.

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